14S1I13 - CLINICAL MICROBIOLOGY

Course specification
Course title		CLINICAL MICROBIOLOGY
Acronym		14S1I13
Acronym
Module
Type of study
Lecturer (for classes)		prof. dr Nenad Milić prof. dr Dejan Krnjaić prof. dr Dušan Mišić prof. dr Jakov Nišavić prof. dr Marina Radojičić doc. dr Andrea Radalj
Lecturer/Associate (for practice)		prof. dr Nenad Milić prof. dr Dejan Krnjaić prof. dr Dušan Mišić prof. dr Jakov Nišavić prof. dr Marina Radojičić doc. dr Andrea Radalj
Lecturer/Associate (for OTC)
ESPB		10.0	Status
Condition		Enrolled in 1st semester
The goal		Acquiring knowledge on morphological, structural, physiological and antigenic characteristics of patogenic microorganisms as well as the methods of their isolation and identification.
The outcome		Students should be able to correctly perform the sampling of suspected material, determine the morphological traits of microorganisms, perform sterilization methods and apply adequate biosafety measures during work with infectious agents. Students should also be capable to perform isolation of microorganisms in pure culture, apply various standard microbiological methods, examine their susceptibility to antibiotics as well as to determine their antigenic characteristics by using standard immunological methods. Students should be acquainted with the application of standard virological methods, i.e. isolation of viruses in cell culture and chicken embryos and their confirmation by various standard methods.
Contents
Contents of lectures		Bacterial physiology; Nutrition and growth of bacteria; Bacterial metabolism; Assimilation activities of bacteria; Infection; Toxins; Genetics of microorganisms; Morphological, structural and physiological characteristics of the most important genera and species of bacteria, fungi and yeasts in veterinary medicine; Definition of viruses; Morphological and structural traits of viruses; Chemical composition of viruses; The influence of physical and chemical factors on viruses; Virus-cell interactions; Viral etiology of tumors; Basic characteristics of viral infections; Morphological and structural characteristics of the most important viruses in veterinary medicine; Viral antigens; The classification of viruses;
Contents of exercises		Sampling and processing of material; Staining techniques in microbiology; Isolation of microorganisms in pure culture; Determination of biochemical traits of microorganisms - standard and automated methods; Biological survey; Serology; Techniques of viral isolation in cell culture and chicken embryos; Immunofluorescence;Virus-neutralization tests; Methods of hemagglutination and inhibition of hemagglutination; The application of molecular methods (PCR) in microbiological diagnostics.
Literature
Milic N., Krnjaic D., Misic D., Nisavic J., Radojicic M. (2017) Microbiology and Immunology. Naucna KMD, Belgrade. Ruzica Asanin, Krnjaic D., Milic N. (2014) Manual of Microbiology and Immunology with practical exercises. Naucna KMD, Beograd. Nisavic J., Milic N., Knezevic Aleksandra (2013) Laboratory diagnostics of viral infections. Naucna KMD, Beograd. Misic D. (2013) Methods of microbiological diagnostics - examination of bacterial resistance to antibiotics. Elit Medica, Beograd. Quinn P.J., Markey B.K., Carter M.E., Donnelly W.J.C., Leonard F.C. (2002) Veterinary Microbiology and Microbial Disease. Blackwell Science, Oxford, UK. (Original title) Hirsh D.C., Zee Y.C. (1999) Veterinary Microbiology. Blackwell Science, Massachusetts, USA. (Original title) Murphy F.A., Gibbs P.E.J., Horzinek M.C., Studdert M.J. (1999) Veterinary Virology. Academic Press, San Diego, California, USA. (Original title)
Number of hours per week during the semester/trimester/year
Lectures	Exercises	OTC	Study and Research	Other classes
4	2	4
Methods of teaching		Theoretical interactive lectures and practical laboratory work.
Knowledge score (maximum points 100)
Pre obligations		Points	Final exam	Points
Activites during lectures		10	Test paper
Practical lessons		20	Oral examination	50
Projects
Colloquia
Seminars		20